Fluorescence detection is a natural luminescence reaction, through the reaction of luciferase and ATP, it can detect human cells, bacteria, molds, and food residues, and get the reaction result within 15 seconds. The fluorescence excitation light source must be able to generate sufficient intensity of the fluorophore to absorb the light waves in the band. So far, UVA light sources can include nitrogen molecular lasers, helium-cadmium lasers, high-pressure cadmium lamps, high-pressure xenon lamps and metal halide arc lamps.
UV LED fluorescence detection can be integrated with the original medium of the system itself. As the circulation or flow of the system medium penetrates to the outside of the system, it remains at the leak point, and the corresponding detection lamp is used to detect the outside of the system. The leak point emits very bright fluorescence, thereby indicating the accurate leak point of the system. Fluorescent ink anti-counterfeiting printing technology is a method of using fluorescent ink to print tickets or documents to prevent forgery. This kind of fluorescent ink can show the same fluorescence in a certain part of the printed matter under the irradiation of the purple light. After the fluorescent is irradiated by the purple light, the light-emitting time is short and the light disappears very quickly. After the purple light is turned off, the light is immediately bright disappear.
The working principle of UV LED fluorescence detection is that when certain compounds are irradiated with ultraviolet light, they can be excited to emit fluorescence, and the fluorescence energy emitted can be quantified by measuring the fluorescence energy. Many substances related to life sciences, such as amino acids, amines, vitamins, steroids, and certain metabolic drugs can be detected by fluorescence method. Fluorescence detectors are very useful in the trace analysis of biological samples, especially after the use of fluorescence derivatization agents, which can detect very small amounts of amino acids and peptides.
UV LED fluorescence detection application areas:
PCB field: exposure, curing, printing, drying
FPD field: exposure, curing, printing, cleaning
Semiconductor field: graphics transfer, curing, printing, exposure, cleaning
Printing and dyeing field: drying, curing, printing, exposure, cleaning
Food water treatment field: sterilization and disinfection, deoxygenation, TOC decomposition, food preservation
Medical and biochemical fields: sterilization and disinfection, phototherapy, air cleaning, isotope separation, genetic engineering
Furniture apparel field: drying, curing, printing
Special effect projection field: screen projection, special effect lighting
Criminal investigation detection field: fluorescence analysis, anti-counterfeiting detection, cultural relic identification
Advantages of UV LED fluorescence detection:
1. High sensitivity, UV LED fluorescence detection is the most sensitive liquid chromatography detector, especially suitable for trace analysis.
2. Good selectivity. The relatively small number of molecules with a large quantum efficiency sufficient for detection is the main reason for the high selectivity of the fluorescence detector. In many cases, the high selectivity of UV LED fluorescence detection can avoid the interference of non-fluorescent components, which becomes a unique advantage of fluorescence detection.
3. Wide linear range. When the concentration of the analyte is large, the intensity of the fluorescence detection of the emitted UV LED may decrease as the concentration increases due to the internal filtering effect.
4. Less affected by external conditions.
5. As long as the solvent selected as the mobile phase does not emit fluorescence, UV LED fluorescence detection can be applied to gradient elution.
